Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Teknova 20 x MOPS/SDS Running Buffer. 500mL, Sterile. 8 pack.
MOPS (3-(N-morpholino)propanesulfonic acid) is a Good’s buffer and has a pKa of 7.01, making it an ideal buffer for many biological systems at near-neutral pH. MOPS can be used in cell culture media and biopharma buffer formulations as well as in the purification of antibodies, peptides, proteins and blood components. It is nontoxic to cell culture lines.
Bioworld ADA (N-(2-acetamido)iminodiacetic acid) Buffer, 0.2M, pH 7.0, 100 mL
Synonym:ADA-Na,N-(2-Acetamido)iminodiacetic acid monosodium salt,N-(Carbamoylmethyl)iminodiacetic acid monosodium saltA zwitterionic buffer used in biological applications including protein isolation and characterizationWarning: For laboratory use only.Not fit for agricultural, household, clinical use or human consumption. Appearance: LiquidPH Value: 7.0
![Alkali Scientific PIPES Sodium Salt [Piperazine N,N' bis(2 ethanesulfonic acid) sodium salt], 100 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659846.jpg-250.jpg)
Alkali Scientific PIPES Sodium Salt [Piperazine N,N' bis(2 ethanesulfonic acid) sodium salt], 100 G
PIPES Sodium Salt [Piperazine N,N' bis(2 ethanesulfonic acid) sodium salt] is a high-quality laboratory reagent supplied in a 100g bottle. This sodium salt form of PIPES is used to prepare buffers with precise pH control, particularly in biological and biochemical research. The sodium salt version is more soluble in water than the free acid form, making it ideal for high-concentration buffer solutions. It is widely used in protein purification, enzymatic assays, and molecular biology applications that require pH stability under varying conditions. The 100g pack size is suitable for small-scale experiments, and the reagent provides a reliable means of controlling pH in critical laboratory workflows.
Medchemexpress LLC MOPS sodium salt | 71119-22-7 | 99.8% | 500 G
MOPS sodium salt is a buffering agent used in biology. MOPS buffer maintains the pH of mammalian cell culture media. It significantly improves the thermal stability of BSA. MOPS sodium salt is relatively safe for short-term experiments, but it may significantly reduce cell viability after 4 hours, so it is recommended to limit the experiment duration to 4-12 hours.
- Purity: 99.8%
- Appearance: Solid
- Color: White to off-white
- Storage: 4°C, sealed, away from moisture. In solvent: -80°C for 6 months; -20°C for 1 month (sealed, away from moisture).
- Solubility in H2O: 100 mg/mL (needs ultrasonic)
- Solubility in DMSO: 50 mg/mL (needs ultrasonic; use newly opened DMSO)
Alkali Scientific Murashige & Skoog Medium with MES Buffer and Vitamins, 49 Grams of Powder, Makes 10 Liters of Solution
Murashige & Skoog Medium with MES Buffer and Vitamins (10 liters, 49 grams of powder) is designed for plant tissue culture applications that require large volumes of prepared solution. This medium is enriched with essential nutrients, growth regulators, and MES buffer, which helps to stabilize the pH during plant tissue growth. The formula ensures optimal growth conditions for plant cells, tissues, and organs in vitro, making it ideal for shoot multiplication, regeneration, and propagation experiments. It is commonly used in commercial plant propagation, agricultural research, and plant breeding. The large 10-liter yield makes this medium suitable for labs and research facilities with higher throughput needs.
eMolecules 58884-35-8 | 4-(6-methyl-1,2,4,5-tetrazin-3-yl)phenol | Broadpharm | MFCD24716961 | 188.190 | C9H8N4O | 98.000 | Cc1nnc(nn1)-c1ccc(O)cc1 | 1g | 349512533
4-(6-methyl-1,2,4,5-tetrazin-3-yl)phenol | Broadpharm | 58884-35-8 | MFCD24716961 | 188.190 | C9H8N4O | 98.000 | Cc1nnc(nn1)-c1ccc(O)cc1 | 1g | 349512533
![Alkali Scientific PIPES Sodium Salt [Piperazine N,N' bis(2 ethanesulfonic acid) sodium salt], 250 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659773.jpg-250.jpg)
Alkali Scientific PIPES Sodium Salt [Piperazine N,N' bis(2 ethanesulfonic acid) sodium salt], 250 Grams
PIPES Sodium Salt (Piperazine N,N' bis(2 ethanesulfonic acid) sodium salt) is a high-purity biochemical reagent provided in a 250G pack. It is widely used as a buffering agent in biological and biochemical assays due to its excellent stability in a wide pH range. The sodium salt form of PIPES is ideal for maintaining stable pH conditions in sensitive biological environments. It is commonly used in molecular biology, protein analysis, and enzyme assays, as it does not interfere with biological systems. PIPES Sodium Salt is also used in chromatographic applications and as a mobile phase additive in high-performance liquid chromatography (HPLC).
![Alkali Scientific BES [N,N bis(2 Hydroxyethyl) 2 aminoethanesulfonic acid], 25 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659860.jpg-250.jpg)
Alkali Scientific BES [N,N bis(2 Hydroxyethyl) 2 aminoethanesulfonic acid], 25 G
BES (N,N bis(2 Hydroxyethyl) 2 aminoethanesulfonic acid) is a biochemical reagent used as a buffering agent in laboratory applications. The 25g quantity is ideal for smaller experiments or laboratories with limited storage space. BES is known for its effective pH buffering capabilities, making it an essential component in protocols that require precise pH control, such as protein purification, electrophoresis, and enzyme assays. It helps maintain the stability of biochemical reactions and is frequently used in molecular biology, biochemistry, and medical research where consistent pH levels are critical for experimental success. The small size is ideal for smaller-scale experiments or specialized applications.
Medchemexpress LLC Rasagiline-13C3 mesylate | 1391052-18-8 | 270.32 | 1 MG
Rasagiline-13C3 mesylate is a deuterium-labeled Rasagiline mesylate, which acts as a highly potent, selective, and irreversible mitochondrial monoamine oxidase (MAO) inhibitor. It exhibits IC50s of 4.43 nM and 412 nM for rat brain MAO B and A activity, respectively.
- Used as a tracer.
- Internal standard for quantitative analysis by NMR, GC-MS, or LC-MS.
- Incorporates stable heavy isotopes into drug molecules for quantitation.
- Deuteration can affect the pharmacokinetic and metabolic profiles of drugs.
Alkali Scientific Murashige & Skoog Medium with MES Buffer and Vitamins, 122.5 Grams of Powder, Makes 25 Liters of Solution
Murashige & Skoog Medium with MES Buffer and Vitamins (25 liters, 122.5 grams of powder) provides a high-volume solution for large-scale plant tissue culture operations. This formulation includes MES buffer, which maintains a stable pH and ensures optimal growth conditions for plant cells and tissues. The medium supports a variety of plant growth applications, including shoot multiplication, organogenesis, and regeneration. The addition of vitamins and essential nutrients promotes cell division and tissue development in controlled laboratory settings. This medium is ideal for research labs, agricultural facilities, and commercial propagation projects where large volumes of plant tissue culture medium are required for successful plant growth.
![Alkali Scientific PIPES [Piperazine N N' bis(2 ethanesulfonic acid)], 500 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659953.jpg-250.jpg)
Alkali Scientific PIPES [Piperazine N N' bis(2 ethanesulfonic acid)], 500 G
PIPES (Piperazine N,N' bis(2 ethanesulfonic acid)) is a biochemical reagent commonly used to prepare buffer solutions that maintain a stable pH environment in various laboratory experiments. This 500g formulation is perfect for high-volume applications in research involving protein purification, enzyme assays, and microbiological cultures. PIPES provides reliable pH stability around 7.0 and is widely used in studies involving proteins, cells, and biological macromolecules. Its high buffering capacity makes it ideal for maintaining consistent experimental conditions during cell growth, enzymatic reactions, and protein analysis, ensuring accurate and reproducible results.